FIG. 5.

Induction of expression in vegetative growing cells of sspG-lacZ in cells expressing ς^K (A) or sspJ-lacZ in cells expressing ς^F (B) or ς^G (C). Cells of strains IB502 (sspG-lacZ Pspac-ς^K) (A), IB480 (sspJ-lacZ Pspac-ς^F) (B), or IB481 (sspJ-lacZ Pspac-ς^G) (C) were grown at 37°C in 2× YT medium. An OD₆₀₀ of 0.25 (time 0 in the figure), the cultures were divided in half, one-half was made 2 mM in IPTG, incubation was continued, and samples were taken from both cultures for assay of β-galactosidase. ■, without IPTG; □, with IPTG. Note the different scales in panels B and C.