FIG. 7.

Primer extension analysis of the sspJ transcription start site. RNA from cells of strain IB465 was isolated 5 h into sporulation, and primer extension products were obtained and analyzed as described in Materials and Methods. The primer used is prot3-50, which anneals only to sspJ mRNA. Lanes a, g, c, and t are DNA sequencing reactions with the same primer and plasmid pIB460; lane 1 is the primer extension reaction with sporulating-cell RNA. The primer extension product is marked with an arrow, and the transcription start site on the sspJ upstream sequence to the right of the figure is marked with a square. Note that the sequence shown is the complement of the mRNA sequence.