. 1998 Dec;180(24):6704–6712. doi: 10.1128/jb.180.24.6704-6712.1998

TABLE 1.

B. subtilis strains used in this study

Strain	Relevant genotype or phenotype	Source or reference
PS482^a	ΔsspA ΔsspB ΔsspE Cm^rb (α⁻β⁻γ⁻)	9
PS832^a	Wild-type derivative of strain 168	Laboratory stock
PY79^c	Wild type	47
IB464^a	sspG::sspG-lacZ Cm^r	pIB454→PS832
IB465^a	sspJ::sspJ-lacZ Cm^r	pIB452→PS832
IB488^a	ΔsspG-yurS::spc Sp^r ^d	This work
IB490^a	ΔsspJ::spc Sp^r	This work
IB466^c	spoIIAC sspG::sspG-lacZ Cm^r	IB464→SC1159
IB467^c	spoIIGB sspG::sspG-lacZ Cm^r Em^re	IB464→SC137
IB468^c	spoIIIG sspG::sspG-lacZ Cm^r	IB464→SC500
IB469^c	spoIVCB sspG::sspG-lacZ Cm^r Em^r	IB464→SC64
IB470^c	sspG::sspG-lacZ Cm^r	IB464→PY79
IB471^c	spoIIAC sspJ::sspJ-lacZ Cm^r	IB465→SC1159
IB472^c	spoIIGB sspJ::sspJ-lacZ Cm^r Em^r	IB465→SC137
IB473^c	spoIIIG sspJ::sspJ-lacZ Cm^r	IB465→SC500
IB474^c	spoIVCB sspJ::sspJ-lacZ Cm^r Em^r	IB465→SC64
IB475^c	sspJ::sspJ-lacZ Cm^r	IB465→PY79
IB502^c	sspG::sspG-lacZ pspac-sigK sigKΔ19 Cm^r Sp^r	IB470→BZ536
IB480^c	sspJ::sspJ-lacZ spoIIIG [pSDA4] Cm^r Km^rf	pSDA4→IB473
IB481^c	sspJ::sspJ-lacZ spoIIIG [pDG298] Cm^r Km^r	pDG298→IB473
IB492^g	sspG::sspG-lacZ trpC2 Cm^r	IB464→SG38
IB494^g	gerE36 sspG::sspG-lacZ trpC2 Cm^r	IB464→522.2
IB498^g	SpβcotA-lacZ trpC2 Cm^r Em^r	Spβ::cotA-lacZ→SG38
IB511^a	ΔsspG-yurS::spcΔ sspA ΔsspB ΔsspE Cm^r Sp^r	IB488→PS482
IB515^a	ΔsspJ::spc ΔsspA ΔsspB ΔsspE Cm^r Sp^r	IB490→PS482
SC64^c	spoIVCB Em^r	S. Cutting
SC137^c	spoIIGB Em^r	S. Cutting
SC500^c	spoIIIGΔ1	S. Cutting
SC1159^c	spoIIAC1	S. Cutting
BZ536^c	Pspac-PsigK sigKΔ19 Sp^r	L. Kroos
522.2^g	gerE36 trpC2	L. Kroos
SG38^g	trpC2	J. Errington

Genetic background is PS832.

Cm^r, chloramphenicol (5 μg/ml) resistance.

Genetic background is PY79.

Sp^r, spectinomycin (100 μg/ml) resistance.

Em^r, erythromycin (1 μg/ml) resistance.

Km^r, kanamycin (10 μg/ml) resistance.

Derivatives of B. subtilis strain CU267 (originally obtained from S. A. Zahler).