TABLE 2.

Characterization of minor SASP in B. subtilis spores^a

Protein bandb	N-terminal sequence	Gene identified in B. subtilis genomec	No. of amino acid residuesd	New gene designation
1	SENRHENEENRRDA-	3353125 to 3353268	47	sspG
2	TKNQNQYQQPN-	tlpe	82	tlp
3	MNIQRAKEIVES-	yfjU	59	sspH
4	ASRNKLVVPGVE- and MDLNLRHAVIAN-	sspD	63	sspD
		ysfA	71	sspI
5	AQQSRSRSNNNN-f	sspC	71	sspC
6	GFFNKDKGKRSE-	3420667 to 3420530	45	sspJ
7	VRNKEKGFPYEN-	927771 to 927622	49	sspK
8	VKRKANHVINGM-	cotK	47	cotK
9	MKKKDKGRLTGG-	2310101 to 2310226	42	sspL
10	MKTRPKKAGQQK-	2338912 to 2339013	34	sspM

^aProtein bands were generated and sequenced as described in Materials and Methods. 

^bAs labeled in Fig. 1, lane e. 

^cGene names are as in the B. subtilis genomic sequence available on the World Wide Web at www.pasteur.fr/Bio/SubtiList.html. Numbers given are the locations in the genome of regions coding for proteins not previously identified as ORFs. The first and second numbers are those of the first nucleotide of the start codon and the last nucleotide of the coding sequence, respectively. 

^dResidues in the intact protein; the N-terminal methionine of a number of the proteins is removed posttranslationally. 

^eThis was the major sequence observed; however, there were also significant amounts of the same sequence but with an N-terminal methionine (∼20% of the total) or beginning at the first lysine residue (∼30% of the total). 

^fSequence from reference 46.