KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit monoclonal anti-gamma H2A.X (1:1000) | Abcam | Cat# ab81299 |
| Rabbit polyclonal anti SHMT2 (1:1000) | Abcam | Cat# ab224427 |
| Mouse monoclonal anti-beta Actin (1:5000) | Abcam | Cat# ab6276 |
| Mouse monoclonal anti GAPDH (1:1000) | Santa Cruz | Cat# sc-365062 |
| Donkey anti-Rabbit IgG Secondary Antibody (1:20000) | LI-COR Biosciences | Cat# 926–68073 |
| Donkey anti-Mouse IgG Polyclonal Antibody (1:20000) | LI-COR Biosciences | Cat# 926–32212 |
| Chemicals, peptides, and recombinant proteins | ||
| SHIN1 | MedChemExpress | Cat# HY-112066A |
| 13C2 glycine | Sigma Aldrich | Cat# 283827 |
| Acetaminophen | Sigma-Aldrich | Cat# A7085 |
| Glycine | Sigma-Aldrich | Cat# G7126 |
| D-alanine | Sigma-Aldrich | Cat# A7377 |
| N-acetyl-L-cysteine | Sigma-Aldrich | Cat# A7250 |
| RIPA Lysis buffer | ThermoFisher Scientific | Cat# 50–103-5430 |
| Protease inhibitor Cocktail | Merck | Cat# 539134 |
| Bradford Protein Assay | Bio-Rad | Cat# 5000205 |
| Methanol, LC-MS Grade | Merck | Cat# 106035 |
| Acetonitrile, LC-MS Grade | Merck | Cat# 100029 |
| Paraformaldehyde | ThermoFisher Scientific, | Cat# AAJ61899AK |
| Tissue Freezing Medium | Leica | Cat# 14020108926 |
| Oil red O staining (ORO) | Sigma-Aldrich | Cat# 01391 |
| Hematoxylin and eosin (H&E) | Vector Laboratories | Cat# H-3502 |
| Aqueous mounting media | Leica | Cat# 94–9990402 |
| 13C3 serine | Cambridge Isotope Laboratories | Cat# CLM-1574-H |
| Ammonium carbonate, LC-MS Grade | ThermoFisher Scientific | Cat# 10785511 |
| Ammonium hydroxide, LC-MS Grade | ThermoFisher Scientific | Cat# 15547049 |
| Formic Acid, LC-MS Grade | Merck | Cat# 533002 |
| DMEM: F12 media | Sartorius | Cat# 01–170-1A |
| Fetal bovine serum | ThermoFisher Scientific | Cat# 10270106 |
| Penicillin Streptomycin | Sartorius | Cat# 03–031-5B |
| Dexamethasone | Sigma-Aldrich | Cat# D4902 |
| ITS-G | ThermoFisher Scientific | Cat# 41400045 |
| Glutamine | Sartorius | Cat# 03–020-1A |
| Earle’s Balanced Salt Solution | Sartorius | Cat# 02–0101-1A |
| Vitamin solution | Sartorius | Cat# 01–326-1B |
| Non-essential amino acids | Sartorius | Cat# 01–325-1B |
| Glutathione | Sigma-Aldrich | Cat# G6013 |
| Ammonium metavanadate | Sigma-Aldrich | Cat# 204846 |
| Manganese chloride | Sigma-Aldrich | Cat# 244589 |
| Ascorbic acid | Sigma-Aldrich | Cat# A4403 |
| Palmitic acid | Sigma-Aldrich | Cat# P0500 |
| Oleic acid | Sigma-Aldrich | Cat# O1383 |
| BSA | Sigma-Aldrich | Cat# A3294 |
| BODIPY 493/503 | ThermoFisher Scientific | Cat# D3922 |
| DAPI | Sigma Aldrich | Cat# D9542 |
| Dihydroethidium | Sigma Aldrich | Cat# D7008 |
| Hoechst | ThermoFisher Scientific | Cat# R37165 |
| TRIzol | Sigma Aldrich | Cat# 93289 |
| Nitrocellulose membrane | Bio Rad | Cat# 1704271 |
| Critical commercial assays | ||
| Alanine Transaminase Assay Kit | ThermoFisher Scientific | Cat# 50–489-244 |
| Aspartate Transaminase Assay Kit | ThermoFisher Scientific | Cat# 50–489-248 |
| LDH-Cytotoxicity Assay Kit | Abbot GmBH, | Cat# 2P56–22 |
| Triglyceride Colorimetric Assay Kit | Cayman Chemical | Cat# 10010303 |
| Direct-ZolTM RNA MiniPrep Plus kit | Zymo Research | Cat# 11–331PB |
| cDNA Reverse Transcription Kit | ThermoFisher Scientific | Cat# 4368814 |
| Fast SYBR™ Green Master Mix | ThermoFisher Scientific | Cat# 4385612 |
| Cholesterol kit | Abbot | Cat# 04S9230 |
| Deposited data | ||
| Source Data (Data S1 & S2) | This paper | |
| Experimental models: Cell lines | ||
| AML12 cells | ATCC | Cat# CRL-2254 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6JOlaHsd | Envigo | |
| Mouse: Alb-cre mice (C57BL/6J background) | The Jackson Laboratory | Cat# 003574 |
| Mouse: Shmt2fl/fl | This paper | |
| Oligonucleotides | ||
| Primer sequences for Shmt2 genotyping: Forward 5’-GGGCCAGGAGAGTTTATCAGACAG-3’ Reverse 5’-ACAGGCTGCAGGGGTGAGACG-3’ | This paper | |
| Primer sequences for Shmt2 genotyping: Forward 5’- CCTTACGTACGGTCCGTCTTCCAC-3’ Reverse 5’-CCCCCCGCGTTCCCCTCT-3’ | This paper | |
| Primers for Hmox1: Forward 5’- AGGTACACATCCAAGCCGAGA- 3’ Reverse 5’- CATCACCAGCTTAAAGCCTTCT- 3’ | 47 | |
| Primers for Nqo1: Forward 5’- TCTCTGGCCGATTCAGAGTG- 3’ Reverse 5’- CCAGACGGTTTCCAGACGTT- 3’ | 48 | |
| Primers for Gapdh: Forward 5’- AGACGGCCGCATCTTCTT- 3’ Reverse 5’- TTCACACCGACCTTCACCAT- 3’ | 48 | |
| Software and algorithms | ||
| Compound Discoverer | ThermoFisher Scientific | Version 3.3 https://www.thermofisher.com/order/catalog/product/OPTON-31061 |
| GraphPad Prism | GraphPad Software | Version 8.0 https://www.graphpad.com/features |
| Xcalibur | ThermoFisher Scientific | Version 4.1 https://www.thermofisher.com/order/catalog/product/OPTON-30965 |
| TraceFinder | ThermoFisher Scientific | Version 4.1 https://www.thermofisher.com/order/catalog/product/OPTON-31001 |
| MetaboAnalyst | 49 | Version 5.0 https://www.metaboanalyst.ca/ |
| AutoPlotter | 50 | Version 2.6 https://mpietzke.shinyapps.io/AutoPlotter/ |
| Other | ||
| Standard chow diet (CD) | Altromin | Cat# 1320 |
| Low fat diet (LFD) | Envigo | Cat# TD.08485 |
| Western diet (WD) | Envigo | Cat# TD.88137 |
| Liquid diet | Bio-Serv | Cat# F1258SP |
| High fat diet | Altromin | Cat# C1084 |
| Disposable animal feeding needles | ThermoFisher Scientific | Cat# 01–208-88 |
| Superfrost Plus slides | ThermoFisher Scientific | Cat#22–037-246 |
| Precellys 24 tissue homogenizer | Bertin Corp | Cat# P002391-P24T0-A.0 |
| Heparin lithium tubes | BD | Cat# 366664 |
| CK14 homogenizing tubes with 1.4 mm ceramic beads | Bertin Corp | Cat# P000926-LYSK0-A |
| HPLC glass vials and caps | Agilent | Cat# 8010–0542 |
| Q-Exactive Orbitrap | ThermoFisher Scientific | |
| Orbitrap Exploris 240 Mass Spectrometer | ThermoFisher Scientific | |
| Vanquish Flex ultra-high-performance liquid chromatography (UPLC) system | ThermoFisher Scientific | |
| Ultimate 3000 high-performance liquid chromatography (HPLC) system | ThermoFisher Scientific | |
| ZIC pHILIC column | Merck | Cat# 150460 |
| ZIC-pHILIC guard column | Merck | Cat# 150438 |
| ACQUITY UPLC BEH C18 column | Waters | Cat# 186002352 |