FIG. 4.

Time courses of methylation in vitro. Isolated membranes containing no receptor, Trg, or Tar were incubated with a cellular extract containing ∼1 μM CheR and 50 μM AdoMet at receptor concentrations of ∼5 μM. At the indicated times samples containing ∼50 pmol of receptor were removed, processed, and analyzed as described in Materials and Methods. The data are averages from three independent experiments, and the error bars show standard errors. Points without bars had standard errors within the size of the symbol.