Fig 3.
Effects of intranasal apilimod (Apilimod base: AB, and Apilimod mesylate: AM) treatment on H1N1-infected BALB/c mice. Mice were inoculated intranasally with H1N1 PR8 (1.0 × 105 PFU/mouse), and the animals were sacrificed 5 days post-virus inoculation. Apilimod was treated once daily on day 0 (4 hours before infection), and then on Days 1, 2, and 3. Body weight loss triggered by virus infection (A, B), viral load in lung tissue (C) and nasal tissue (D), and neutrophil accumulation in BALF (E) were evaluated. For viral load, individual data have been plotted as geometric mean. For body weight. mean ± SEM was shown. *P < 0.05, ***P < 0.001 vs H1N1-infected control.