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. Author manuscript; available in PMC: 2024 Jan 10.
Published in final edited form as: Arch Pathol Lab Med. 2020 Nov 1;144(11):1311–1320. doi: 10.5858/arpa.2020-0372-SA

Table:

Details of Validation Cohorts Including Diagnoses and Number or Slide Preparations As Well As the Results of Diagnostic Concordance Evaluation

Nsx Set: Category (No. of Cases) Study Set Number Abbreviated Classification/Diagnosis and Grade (if applicable) H&E, FFPE Special Stains IHC Total Slides SPG Dx QCS (n = 3) SPG SS/ IHC QCS (n = 3) STG Dx QCS (n = 2) STG SS/ IHC QCS (n = 2) Diagnostic Image Quality, Yes/No

CNS/PNS neoplastic (10) Nsx-01 Central neurocytoma, WHO grade II 1 0 4 (SYN, NeuN, GFAP, NF) 5 0 0 0 0 Yes
Nsx-02 Ependymoma, WHO grade II 1 0 3 (GFAP, EMA, NF) 4 0 0 0 0 Yes
Nsx-03 Glioblastoma, WHO grade IV 1 0 0 1 0 0 0 0 Yes
Nsx-04 Oligodendroglioma, IDH-mutant and 1 p/19q codeleted, WHO grade II 1 0 5 (mlDHl, ATRX, P53, GFAP, NF) 6 0 0 0 0 Yes
Nsx-05 Myxopapillary ependymoma, WHO grade I 1 1 (Al Bl) 1 (GFAP) 3 0 0 0 0 Yes
Nsx-07 Medulloblastoma, WHO grade IV 1 0 0 1 0 0 0 0 Yes
Nsx-09 Hemangioblastoma, WHO grade I 1 0 0 1 0 0 0 0 Yes
Nsx-10 Schwannoma, WHO grade I 1 0 0 1 0 0 0 0 Yes
Nsx-13 Adamantinomatous craniopharyngioma, WHO grade I 1 0 0 1 0 0 0 0 Yes
Nsx-15 Secretory meningioma, WHO grade I 1 0 0 1 0 0 0 0 Yes
CNS/PNS nonneoplastic (3) Nsx-06 Arteriovenous malformation 1 1 (VVG) 0 2 0 0 0 0 Yes
Nsx-08 Neurocysticercosis 1 0 0 1 0 0 0 0 Yes
Nsx-11 Cavernous malformation (cavernoma) 1 1 (GT) 0 2 0 0 0 0 Yes
Brain autopsy neoplastic (1) Nsx-14 Intravascular B-cell lymphoma 1 0 1 (CD20) 2 0 0 0 0 Yes
Brain autopsy nonneoplastic (1) Nsx-12 Coccidioidal meningitis 1 1 (GMS) 0 2 0 0 0 0 Yes
Ophthalmic neoplastic (2) Oph-1 Uveal melanoma 1 0 0 1 0 0 0 0 Yes
Oph-3 Invasive squamous cell carcinoma 1 0 0 1 0 0 0 0 Yes
Ophthalmic nonneoplastic (3) Oph-2 Hidrocy stoma 1 0 0 1 0 0 0 0 Yes
Oph-4 Epidermal inclusion cyst 1 0 0 1 0 0 0 0 Yes
Oph-5 Lipogranuloma (chalazion) 1 0 0 0 0 0 0 Yes
Total 20 20 4 14 38
Metabolic/mitochondrial myopathy (4) NM-01 GSD/acid maltase deficiency (glycogenosis type II) 0/1 4 (mGT, PAS, ORO, AcP)/0 0/0 5 0 0 0 0 Yes
NM-02 GSD/McArdle disease (glycogenosis type V) 0/1 4 (MP, COX/SDH, ORO, PAS)/0 0/0 5 0 0 0 0 Yes
NM-04 Mitochondrial myopathy 0/1 5 (mGT, MP, COX/ SDH, AMPD, PFK)/1 (MB) 0/0 7 0 0 0 0 Yes
NM-06 Lipid storage disease 0/1 2 (ORO, PAS)/1 (MB) 0/0 4 0 0 0 0 Yes
Dystrophic (1) NM-07 Necrotizing myopathy/dysferlinopathy 0/1 0/0 4 (dysferlin, dystrophins (rod, C-term, N-term))/0 5 0 0 0 0 Yes
Immune/inflammatory (5) NM-03 Inflammatory myopathy/immune myopathy with prominent perimysial pathology 0/1 2 (AcP, AlkP)/0 3 (HLA-I, CD3, CD20)/0 6 0 0 0 0 Yes
NM-05 Inflammatory myopathy/sporadic inclusion body myositis 0/1 0/1 (MB) 4 (HLA-I, CD3, CD20, TDP43)/0 6 0 0 0 0 Yes
NM-08 Immune mediated/macrophagic myofasciitis 0/1 2 (PAS, AcP)/1 (MB) 0/0 4 0 0 0 0 Yes
NM-09 Immune-mediated neuropathy with axonopathy 1/0 0/2 (MB, teased nerve fiber preparation) 0/1 (CD 3) 4 0 0 0 0 Yes
NM-10 Immune-mediated/vasculitis with axonopathy and neurogenic atrophya 3/1 0/1 (MB) 1 (CD3)/3 (CD3, CD68, NF) 9 0 0 0 0 Yes
Total 10 4/9 19/7 12/4 55

Abbreviations: AcP, acid phosphatase; Al Bl, Alcian blue; AlK P, alkaline phosphatase; AMPD, myoadenylate deaminase; ATRX, a-thalassemia/mental retardation, X-linked; CNS, central nervous system; COX/SDH, cytochrome c oxidase/succinate dehydrogenase; DX, diagnosis; EMA, epithelial membrane antigen; FFPE, formalin fixed, paraffin embedded; Fzn, frozen; GFAP, glial fibrillary acidic protein; GMS, Grocott methenamine silver stain; GSD, glycogen storage disease; GT, Gomori trichrome; H&E, hematoxylin-eosin; HLA-I, human leukocyte antigen class I; IHC, immunohistochemical stains; MB, methylene blue; mGT, modified Gomori trichrome; mIDH, mutant isocitrate dehydrogenase; MP, myophosphorylase; NeuN, neuronal nuclear protein; NF, neurofilament; NM, neuromuscular; Nsx, neurosurgical; ORO, Oil Red O; PAS, periodic acid-Schiff; PFK, phosphofructokinase; PNS, peripheral nervous system; QCS, quality concern score; SPG, study pathologist group; SS, special stains; STG, study trainee group; SYN, synaptophysin; TDP43, TAR DNA-binding protein 43; VVG, Verhoeff-Van Gieson stain; WHO, World Health Organization.

a

Includes slides additionally provided per request of 1 SPG member (2 additional H&E stains and CD3 and CD68 IHC).