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S1 nuclease protection analysis of transcripts expressed from three btuB-lacZ fusions. RNA was extracted from cells of strain RK5173 carrying the plasmids with the indicated btuB-lacZ fusions. Cells were grown as indicated in the absence or presence of 1 μM Ado-Cbl and extracted RNA was hybridized with 32P-labeled probe A. In all assays, a constant amount of labeled probe complementary to the bla gene transcript was included. The sizes of the protected DNA fragments are indicated.
