TABLE 1.
List of bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristics | Source or reference |
|---|---|---|
| Strains | ||
| CA244 | lacZ trp relA spoT | 10 |
| JM109 | Promega | |
| MC1061 | F−araD139 galU galK ΔlacX74 Δ(ara-leu)7697 rpsL hsdR | J. Beckwith |
| MG1655 | Wild type | 24 |
| MG1693 | F−thyA715λ− | 2 |
| RK3513 | BL21(DE3) btuB+ zij::Tn10 | This study |
| RK5173 | Δ(argF-lac)U169 araD139 fib5301 ptsF25 relA1 rpsL150 rbsR22 deoC1 gyrA219 non-9 metE70 | 13 |
| SK5665 | MG1693 rne-1 | 2 |
| SK6867 | MC1061 rnc-105 Tcr | 2 |
| SK7621 | MC1061 Δrnc38::Km | 2 |
| SK7669 | MG1693 rne-3071 | 2 |
| TX2817 | MG1655 hfq2::ΩKm | 24 |
| Plasmids | ||
| pAG1 | pBR322 btuB+; Ap | |
| pRS414 | Translational lac fusion vector; Ap | 22 |
| pRS415 | Transcriptional lac fusion vector; Ap | 22 |
| pEB450X | pRS415 with −60 to +450 of btuB-lacZ; Ap | 7 |
| pEB450L | pRS414 with −60 to +450 of btuB-lacZ; Ap | 7 |
| pEB315X | pRS415 with −60 to +315 of btuB-lacZ; Ap | 7 |
| pEB270X | pRS415 with −60 to +270 of btuB-lacZ; Ap | 7 |
| pXN5 | pBluescript SK+ with +1 to +450 of btuB; Ap | This study |
| pXN17 | pRS415 with T7P, +1 to +450 of btuB-lacZ; Ap | This study |
| pXN18 | pRS414 with T7P, +1 to +450 of btuB-lacZ; Ap | This study |
| pXN24 | pRS415 with lacP, +1 to +450 of btuB-lacZ; Ap | This study |
| pXN25 | pRS414 with lacP, +1 to +450 of btuB-lacZ; Ap | This study |
| pXN26 | pRS415 with blaP, +1 to +450 of btuB-lacZ; Ap | This study |
| pXN27 | pRS414 with blaP, +1 to +450 of btuB-lacZ; Ap | This study |