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. 2023 Dec 21;16(1):48. doi: 10.3390/cancers16010048

Figure 5.

Figure 5

miR-410 regulates epithelial-to-mesenchymal transition in prostate cancer. (A) Western blot analyses of indicated proteins in miR-CON/miR-410 transfected LNCaP cells. GAPDH was used as a loading control. (B) Schematic representation of 3′ UTR of SNAI1 showing the complementarity of two putative binding sites, SNAI1-1 (Site 1) and SNAI1-2 (Site 2), with miR-410 seed sequence. (C) Western blot analyses of SNAIL expression in miR-CON/miR-410 transfected LNCaP cells. GAPDH was used as a loading control. (D) Luciferase reporter assay in LNCaP cells transfected with miR-CON/miR-410 and co-transfected with control pmiR-Glo or SNAI1-1 or SNAI1-2 3′ UTR constructs. Firefly and Renilla luciferase activities were measured, and the relative luciferase activities were plotted. (E) Western blot analyses of indicated proteins in miR-CON/miR-410 transfected PC3 and C42B cells. GAPDH was used as a loading control. (F) Western blot analyses of MMP10 in C42B, PC3 or LNCaP cells. GAPDH was used as a loading control. All Western blot images were analyzed by Image J Version 1.54d and relative band intensities were calculated and are shown below the blots. * denotes p < 0.05.