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. 2023 Dec 27;16(1):139. doi: 10.3390/cancers16010139

Figure 3.

Figure 3

CARM1 is involved in the activation of the Wnt/β-catenin signaling pathway regulated by RBM5. (A) J82 cells were transfected with LV-oeCARM1 or LV-Con, and western blotting was performed to examine the protein expression of p-GSK3β, GSK3β, and β-catenin. (B) Quantitative analysis of (A). (C) T24 cells were transfected with LV−shCARM1 or control lentivirus, after which p-GSK3β, GSK3β, and β-catenin protein levels were assessed via western blotting. (D) Quantitative analysis of (C). (E) T24 cells were transfected with LV−shRBM5 or LV−shCARM1 alone or in combination; afterward, western blotting was performed to detect the protein expression of p-GSK3β, GSK3β, and β-catenin. (F) Quantitative analysis of (E). (G) T24 cells were transfected with LV-shCARM1 or LV-shCon and then treated with XAV939. The CDK4, p-GSK3β, GSK3β, and β-catenin protein levels were measured via western blotting. (H) Quantitative analysis of (H). All data were derived from three independent experiments and expressed as the mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. their corresponding controls.