Figure 7.

Blocking the RBM5/CARM1/PRKACA axis reduces the proliferation of in vivo bladder cancer (BC) cells. (A) T24 cells were stably knocked down with CARM1 or PRKACA, alone or in combination, and then implanted into nude mice for 21 days to establish xenograft tumors. The size of the xenograft tumors in each group is shown. (B,C) Xenograft tumor volumes and wet weight were determined after tumor resection. (D) The expression of CDK4, β-catenin, and CARM1 in the xenograft tumors was detected via immunohistochemistry. Scale bar = 100 μm. (E,F) Western blotting was used to assess the expression of p-GSK3β, CDK4, and β-catenin in the xenograft tumor tissue. All data were derived from three independent experiments and expressed as the mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01, *** p < 0.001 vs. their corresponding controls.