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. 2023 Dec 28;16(1):159. doi: 10.3390/cancers16010159

Figure 2.

Figure 2

scRNAseq of one HCA and three NBNC-HCC samples. (a) The tSNE plot of the total for 7612 cells (NBNCHCC1; 1897 cells, NBNCHCC2; 2001 cells, NBNCHCC3; 2123 cells, and HCA; 1591 cells). The merged cells were divided into 19 clusters. (b) Cell types and sample-identified tSNE plots. The majority of the cells in NBNCHCC1 (red), NBNCHCC2 (green), and NBNCHCC3 (sky blue) were CD8A+ macrophages (MP, cluster #3, #6), GZMB+NKG7+ Tcell (cluster #1), or FN1highFABP1+cancer cells (clusters #4 and #5). (c) Heat map analysis of the four samples. The color in the heatmap from yellow to violet reveals gradual differences in expression intensity from high to low. The letters listed on the right side of the heatmap indicate representative gene groups or gene symbols. MP1, macrophage cluster 1; Mes, mesenchymal cells; Endo, endothelial cells; Mast: Mast cells; and Pro, MYChigh proliferated cells. (d) Feature plots of representative I-HCA makers, SAA1, SAA2, and CRP. The color bar, from red to blue through yellow, reveals gradual expression intensity differences from high to low through the middle. (e) PLA2G2A features and violin plots. (f) Ratio of double-positive cells to ALB and each gene for total cells in HCA clusters #15 or #18. Violet bars: Cluster #15; red bars: Cluster #18. (g) Gene–gene correlation plots associated with PLA2G2A in clusters #15 and #18. The x-axis indicates the coefficient of determination (R2).