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. 2023 Dec 21;13(1):24. doi: 10.3390/cells13010024

Figure 1.

Figure 1

Extracellular vesicles isolated via size-exclusion chromatography. EVs were isolated from AD-1000 bioreactors via SEC with 20 0.5 mL fractions collected, with CD81-positive particles eluting in fractions 5–10 and later elution of other proteins ((A), n = 3). Pooling fractions 5–10 provided robust EV stocks with a median particle size of ~80 nm ((B), n = 3). Pools were positive for CD9, CD81 and CD63 using DELFIA ELISA ((C), n = 3), and the presence of double-membraned particles was confirmed using transmission electron microscopy (D).