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. 2023 Dec 20;13(1):17. doi: 10.3390/cells13010017

Figure 3.

Figure 3

Analysis of SPM receptors ChemR23 and FPR2 in histological sections: (A) Exemplary immunofluorescence image of a bursa sample from the severe disease group showing single staining channels for Dapi, ChemR23, FPR2 and CD45, as well as channel overlap (merged), and the respective result after image analysis (Makro). Scale bars represent 50 µm. (B) Quantification of immunofluorescence staining of total ChemR23, FPR2 and CD45 (single- + double- + triple-positive staining area) in bursae of intact controls (n = 8), moderate disease (n = 7) and severe disease (n = 8) using a self-designed image analysis tool. (C) Distribution of immunofluorescence staining area of single-positive ChemR23, FPR2 and CD45 signal; double-positive ChemR23/FPR2, ChemR23/CD45 and FPR2/CD45 signal; or triple-positive ChemR23/FPR2/CD45 signal. The staining areas are given in % to the total positive staining area.