Figure 2.
Effect of curcumin on liver lipid metabolism in laying hens. (A) The liver weight in laying hens. (B) The liver-to-body ratio. (C) Hepatic TG content. (D) Liver sections stained with HE (40×) in laying hens. (E) Liver sections stained with oil red (10×) in laying hens. (F) Hepatic expression levels of fatty acid synthesis and β-oxidation-related genes in laying hens. (G) Hepatic expression levels of cholesterol transport, synthesis, and degradation-related genes in laying hens. (H) Hepatic expression levels of lipid autophagy-related genes in laying hens. (I) Hepatic expression levels of fatty acid synthesis-related proteins (FASN and SERBP1) in laying hens. β-actin was used as a reference in (F–H), and β-tubulin was used as a reference in (I). CON = control diet group; CUR = control diet supplemented with 200 mg/kg of curcumin. Data are presented as mean ± SEM, n = 14 (A–C), 5 (D,E), or 6 (F–I). * indicated significantly different expression as p < 0.05.
