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. 2023 Dec 19;13(1):2. doi: 10.3390/cells13010002

Figure 5.

Figure 5

EtOH exposure inhibits expression of SERT and synaptic proteins in both synaptosomes and exosomes. (A). SERT and BDNF levels were quantified by ELISA and values expressed in pg/mL, after normalization to the exosomal marker protein CD81. EtOH and SSRI each reduced SERT levels (measured by ELISA; n = 6/group) in synaptosomes, and the effects were additive. (B). EtOH and SSRI each reduced SERT levels in FB-Es (ELISA n = 6/group), and the effect of combined EtOH + SSRI was about the same as that of SSRI alone. (C). Exposure to EtOH reduced BDNF levels in synaptosomes (ELISA; n = 10/group). (D). BDNF levels were reduced in FB-Es of EtOH-exposed cases (p < 0.05; n = 10/group). Graphs show means from triplicate assays +/− SD. (E). Exposure to EtOH reduced synapsin levels compared to controls (n = 10 cases/group) in synaptosomes and in exosomes (F), as assayed by quantitative Western blot, and shown in relative fluorescence units (RFU). * p < 0.05, ** p < 0.01, and *** p < 0.001.