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Cultivation and characterization of metastatic breast cancer (BC) patient-derived organoids (MBC-PDO), originated from malignant pleural effusion (MPE). (A) Schematic overview of isolation of metastatic BC cells from MPEs, as well as cultivation in BME droplets and viral transduction of MBC-PDOs. (B) Brightfield (BF) and fluorescence (FL) images as well as FC analysis of luciferase- and GFP-expressing MCF-7 and MBC-PDO #03, #04, #06, and #07. Scale bar: 200 µm.
