Figure 6.

FC analysis and AdCAR treatment of MBC-PDOs with various LLE-mAbs. Treatments were performed on (A) MBC-PDO #03, (B) MBC-PDO #06, and (C) MBC-PDO #04. AdCAR treatment of MBC-PDOs with (colored bars) and without (gray bars) LLE-mAbs was carried out in an E:T ratio of 1:1 for 72 h. Target cell lysis efficiency was determined by luciferase activity of viable organoids. Data shown represent the mean ± SD of multiple biological replicates (n ≥ 3). Statistical analysis was performed using paired t-test. ns, not significant. * = p ≤ 0.05; ** = p ≤ 0.01.