Table 1.

Statistics and de novo genome assembly data for each set of simulated reads for Escherichia coli O157:H7.

Number of Reads	Reads N50 (b)	Reads N90 (b)	Minimum Read Length (b)	Maximum Read Length (b)	Total Read Length (b)	Assembly	Contig Number in Assembly	Total Length (b)	Genome Coverage	Assembly N50 (b)	Smallest Contig (b)	Largest Contig (b)
10	8849	3483	2582	13,424	72,759	No
50	10,049	5788	208	33,863	422,715	No
75	9233	5346	208	15,536	540,757	No
100	9375	5040	221	17,938	757,508	No
250	9615	5317	217	38,181	1,971,775	No
500	9624	5078	125	43,121	3,878,109	No
750	9700	5476	141	28,563	5,987,151	No			1
1000	9577	5462	141	30,045	7,932,184	Yes	3	185,255	1	117,920	18,111	117,920
2500	9531	5319	94	40,056	19,646,989	Yes	42	2,972,053	3	98,451	26,055	163,256
5000	9426	5232	100	43,248	38,804,704	Yes	27	5,547,668	6	377,010	16,126	614,371
7500	9348	5366	72	45,099	58,630,216	Yes	14	5,525,979	10	1,120,145	12,681	1,327,415
10,000	9407	5282	87	58,762	78,143,078	Yes	9	5,472,930	14	4,015,755	17,274	4,015,755
50,000	9412	5241	81	59,173	388,401,901	Yes	9	5,519,291	70	3,781,995	17,242	3,781,995
75,000	9369	5280	57	59,494	582,224,483	Yes	7	5,489,935	106	4,015,794	17,597	4,015,794
100,000	9380	5280	53	59,146	778,028,874	Yes	7	5,537,798	141	4,013,828	17,324	4,013,828
250,000	9384	5263	49	58,657	1,939,653,454	Yes	6	5,485,282	352	4,029,574	164,347	4,029,574
500,000	9380	5256	50	66,523	3,874,545,789	Yes	4	5,505,839	704	4,013,857	278,922	4,013,857
750,000	9384	5258	48	66,949	5,819,660,420	Yes	5	5,469,961	1058	4397,660	163,836	4,397,660
1,000,000	9389	5271	45	66,154	7,764,565,277	Yes	3	5,479,648	1411	5,054,755	166,955	5,054,755