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Cytotoxicity evaluation of siRNA-complexes at different N/P ratios in (a) MDA-MB 231 cells at siRNA concentration 200 nM and (b) EA.hy926 cells at siRNA concentrations 200 nM and 100 nM. Values are the mean ± S.D. of n = 9 individual samples from three independent experiments; statistical significance was assessed by ordinary one-way ANOVA (a) and two-way ANOVA (b). *—p <0.05, **—p <0.01 compared to corresponding complexes formed at N/P ratio 8/1.
