Figure 3.
ACSE attenuates NO production in LPS-stimulated macrophages. (A) J774 cells were treated with indicated concentrations of ACSE for 24 h. The effects of ACSE on cell viability were measured via WST-1 assay. (B–D) J774 cells were pretreated with indicated concentrations of ACSE and then stimulated with 1 µg/mL of LPS for 24 h. (B) The effect of ACSE on NO production. (C,D) The effects of ACSE on the expression of iNOS and COX-2 mRNAs (C) and proteins (D). * p < 0.05, ** p < 0.01, and # p < 0.001.
