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. 2023 Dec 24;25(1):280. doi: 10.3390/ijms25010280

Figure 6.

Figure 6

Lipofuscin content. Lipofuscin content was quantified from images acquired with a 10× objective lens and analyzed using NIS-Elements BR software (Nikon, Tokyo, Japan). L3 stage synchronized N2 nematodes were treated or untreated with 100 µg/mL T. erecta extract and incubated at 20 °C until the read-out point of the experiment (Young worms: 5 days old; Old worms: 12 days old). Representative images (10× magnification) of lipofuscin quantification by Nikon epi-fluorescence microscope (Eclipse Ni, Nikon, Tokyo, Japan) fitted with a Nikon DS-Ri2 camera (Tokyo, Japan) are shown below the bar charts. Data are expressed as mean ± SD of three independent experiments (n = 3). Lower-case letters, when different, indicate statistically significant differences (p < 0.05). CTL_Young: Young control (untreated worms); T. erecta_young: T. erecta treated worms (Young); CTL_old: Old control (untreated worms); T. erecta_old: T. erecta treated worms (Old).