Figure 7.

Substrate specificity of fucanase FWf5. (A) C-PAGE analysis of the effect of FWf5 on oligosaccharides of different structures. “C”—the control oligosaccharide without the addition of the FWf5. “R”—oligosaccharide incubated with the FWf5 for 24 h at RT. (*)—change in the electrophoretic mobility or disappearance of the oligosaccharide as a result of depolymerization. (B) The proposed sites in the fucoidans that are cleaved by the FWf5 and the structural motifs of the fucoidans that are resistant to the enzyme cleavage. (C) The proposed sugar-binding subsites of the FWf5, with subsites numbered according to the nomenclature [60].