TABLE 1.
Substrate | Km (mM) | Vmax (U · μl−1) | Vmax/Km (U · mM−1 · μl−1) |
---|---|---|---|
ADP-glucose | 1.6 | 1.78 | 1.11 |
UDP-glucose | 3.5 | 3.04 | 0.87 |
GDP-glucose | 2.4 | 0.41 | 0.17 |
The data are representative of at least two independent experiments performed in duplicate. The standard deviations in all determinations did not exceed 10% of the mean. One unit corresponds to the production of one μmol of sucrose per min. Km values are apparent Kms.