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Digestion of pET-MT1 (a) and pET-MT2 (b) plasmid DNA by pP62BP1-encoded REases. The plasmid DNA was extracted from E. coli BL21(DE3). In both cases, digestion by the ScrFI restriction enzyme and the pP62BP1-encoded REases (RE1, RE2) was observed only in the case of the plasmid DNA isolated after the induction of MTase expression. A number of expected digestion products are visible, including the two largest fragments (2462 bp and 695 bp) indicated by red arrows.
