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The sensitivities of lung cancer A549 cells and their chemoresistant sublines with shikonin were characterized by MTT cell survival assay. The sensitivities of A549 cells and their chemoresistant sublines A549/D16 (A) and A549/V16 (B) cells to shikonin (1, 2, 4, 8, and 16 μM) were compared when cells were treated with shikonin alone or combined with DOC (16 nM) or vincristine (VCR, 16 nM) accordingly for 48 h, followed by MTT assay.
