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. 2023 Dec 29;25(1):479. doi: 10.3390/ijms25010479

Figure 4.

Figure 4

Binding selectivity and structural stabilisation of human TTR in diluted plasma samples, by tolcapone, 3-OMT, 1, and 2. Histograms represent the relative abundance of TTR monomer after supplementation of tolcapone analogues at two different concentrations (1 µM, left and 10 µM, right), in comparison to the negative control (DMSO). Data are expressed as mean (SD) of three independent experiments, and a representative western blot result is shown below each graph. Data were analysed by one-way ANOVA and symbols indicate statistical difference between treatments: p > 0.05 (ns), p ≤ 0.05 (*). Only the most relevant comparisons are displayed. Values (%) at concentrations 1 µM are: tafamidis, 47.8 ± 3.6; tolcapone, 28 ± 6.2; 3-OMT, 14.1 ± 1.8; 2, 7.5 ± 0.4; 1, 17.2 ± 2.1; DMSO, 100 ± 0. Values (%) at concentrations 10 µM are: tafamidis, 12.3 ± 3.2; tolcapone, 10.2 ± 3; 3-OMT, 9.4 ± 1.5; 2, 9.2 ± 1.5; 1, 9.5 ± 2.8; DMSO, 100 ± 0.