Figure 4.

Effect of CUR and RES on IFN-γ expression in T lymphocytes and NK cells. IFN-γ expression was assessed by flow cytometry after 96 h of PBMCs treatment with DMSO, CUR and/or RES (5 µM) on (A) resting CD3⁺CD19⁻CD14⁻CD4⁺ T lymphocytes, (B) resting CD3⁺CD19⁻CD14⁻CD8⁺ T lymphocytes, and (C) NK cells. The results are presented as the mean ± SD of the frequency of IFN-γ⁺ cell subsets in PBMCs obtained from six to eight healthy donors. Statistical significance of the effects obtained with CUR and RES, alone or in combination, was calculated with two-tailed unpaired Student’s t test (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001).