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. 2023 Dec 23;25(1):232. doi: 10.3390/ijms25010232

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of CUR and RES on Tregs frequency and IL-10 production. PBMCs were treated for 96 h with DMSO, CUR, and/or RES (5 µM) and analyzed by flow cytometry to assess (A) the frequency of CD4⁺CD25^highCD127^low/neg cells; (B) the frequency of IL-10⁺ Tregs. The results are presented as the mean ± SD of the frequency of cells in PBMCs from eight healthy donors. Statistical significance of the effects obtained with CUR and RES, alone or in combination, was calculated with two-tailed unpaired Student’s t test (* p ≤ 0.05; *** p ≤ 0.001).