Table 2.
Peptide fraction separation liquid chromatography elution gradient table.
| Time (min) | Flow Rate (mL/min) | Mobile Phase A (%) | Mobile Phase B (%) |
|---|---|---|---|
| 0 | 1 | 97 | 3 |
| 10 | 1 | 95 | 5 |
| 30 | 1 | 80 | 20 |
| 48 | 1 | 60 | 40 |
| 50 | 1 | 50 | 50 |
| 53 | 1 | 30 | 70 |
| 54 | 1 | 0 | 100 |
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