Table 3.
List of used growth factors, inhibitors and small molecules for astrocyte differentiation.
| Substance | Company | Concentration | Days |
|---|---|---|---|
| LDN193189 | Sigma-Aldrich, Darmstadt, Germany | 100 nM | 0–11 |
| SB431542 | Abcam, Cambridge, UK | 10 µM | 0–5 |
| Purmorphamine | Tocris, Ellisville, MO, USA | 2 µM | 1–7 |
| SHH C25II | PeproTech, Cranbury, NJ, USA | 100 ng/mL | 1–7 |
| CHIR99021 | Sigma-Aldrich, Darmstadt, Germany | 3 µM | 3–13 |
| bFGF | SCI Store, Moscow, Russia | 20 ng/mL | 13–… |
| EGF | PeproTech, Cranbury, NJ, USA | 20 ng/mL | 13–… |
| CNTF | PeproTech, Cranbury, NJ, USA | 10 ng/mL | 25–… |