Figure 1.

Previous study, data processing and canonical analysis of DEGs in the aged NP ovary. (A) Two C57BL6 female mice cohorts were maintained in nulliparous (NP) and multiparous (MP) breeding regimens from 4 through 16 months old. Short overlapped white arrows depict gestation plus lactation periods in MP mice. (B) Total ovarian RNA from the two conditions was analyzed with Illumina™ beadchip expression microarrays resulting in 177 differentially expressed genes (DEGs) between the NP and MP conditions [13]. (C) Summary of a gene set enrichment analysis (GSEA) of the 112 DEGs of higher expression in NP ovaries, using the MSigDBv7.3 database (details in Methods and Table 1). The gene number associated to each functional theme is indicated in the respective bar, and their identities described in Table 1.