Figure 8.

The impact of various experimental mediums (DMEM vs. NB) on the biosafety profile of edaravone (ED, (A,B)), ebselen (EB, (C,D)), and carnosic acid (CA, (E,F)) in undifferentiated (UN-) and retinoic acid (RA-)-differentiated SH-SY5Y cells. The UN- and RA-SH-SY5Y cells growing overnight in neuroblastoma (DMEM) or neuronal (NB) experimental medium were treated with ED (100 and 500 μM), EB (5–50 μM), or CA (5–25 μM) for 24 h. Cell viability was measured by WST-1 assay. Data after normalization to vehicle-treated cells (100%) are presented as a mean ± SEM. The number of independent experiments (n) is indicated in each graph. *** p < 0.001 vs. vehicle-treated cells; ^& p < 0.05 and ^&& p < 0.01 NB vs. DMEM medium for indicated drug concentration.