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Characteristics of 2D culture cells. (A) Cell morphology of different days (scale bar: 100 μm). (B) Diameter contrast on the third day of differentiation (scale bar: 100 μm). (C,D) Expression of multipotent markers during the first three days after induced differentiation. The expression of multipotent markers OCT4, KLF4, SOX2, and C-MYC was detected by Western Blotting assay. ** p < 0.01; *** p < 0.001; **** p < 0.0001.
