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. 2024 Jan 4;25(1):682. doi: 10.3390/ijms25010682

Figure 3.

Figure 3

Changes in gene expression in 2D cultured porcine kidney organoids. (A) Expression of special markers in different periods. The specific markers for PS cells (MIXL1), IM cells (PAX2), MM cells (SIX2 and PAX2), and UE cells (SIX2 and PAX2) were discovered in the corresponding porcine kidney organoids on days 3, 7, and 14 of 2D culture by immunofluorescence test (MIXL1, PAX2, and SIX2 were all localized to the nucleus) (scale bar: 100 μm). (B) Expression level changes in renal progenitor cell markers at 4 time points (day 0, 7, 14, and 21) of the 2D cultured porcine kidney organoids. The relative expression levels of markers about early nephrons (JAG1, BMP2), nephrogenic stroma (SALL1, ITGA8), ureteral buds (VLDLR, SLCO4C1), and endothelial progenitor cells (KDR, ANGPT2) were measured by real-time PCR. (C) Expression level changes in mature nephron components markers at 4 time points (day 0, 7, 14, and 21) of the 2D cultured porcine kidney organoids. The relative expression levels of markers about podocytes (NPHS1, MAFB), proximal tubules (AQP1, HNF1B), distal tubules (ALDLH1), and endothelial cells (CD31) were measured by real-time PCR. Each value was averaged from three replicates and presented as mean ± SD. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant.