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. 2024 Jan 4;25(1):682. doi: 10.3390/ijms25010682

Figure 7.

Figure 7

Apoptosis and proliferation of porcine kidney organoids and effect of CHIR pulse on the expression of organoid genes expression levels on day 21. (A) a: HE staining of the peripheral parts of porcine kidney organoids. b: HE staining of the core parts of porcine kidney organoids. c: DAPI staining of the nucleus. (B) Proliferation (KI67) and apoptosis (dUTP) in peripheral region of the organoids on day 21 were measured by immunofluorescence test. (C) Proliferation (KI67) and apoptosis (dUTP) in core region of the organoids on day 21 were measured by immunofluorescence test (KI67 and dUTP were localized in the nucleus). (D) Morphology of porcine kidney organoids on day 21 with different diameters. (E) The relative expression levels of markers of nephron progenitor cells (JAG1, SALL1, ANGPT2) and mature nephron components (HNF1B, MAFB, CD31). Each value was averaged from three replicates and presented as mean ± SD. *** p < 0.001; **** p < 0.0001.