FIG. 1.

Genetic maps of the adhE chromosomal regions of L. lactis MG1363 and DB1341. Only relevant restriction sites are shown. E, EcoRI; H, HindIII; N, NsiI; Nd, NdeI; P, PstI; S, Sau3AI. The DNA fragments included in pC1 are shown, indicating the three different Sau3AI DNA fragments that were ligated together under library construction and that included an rRNA gene sequence (rRNA box in clone 1). The position of the consensus E. coli expression signals (Shine-Dalgarno sequence and promoter region) in pC1 is shown as a bent arrow (see also Fig. 2). The fragment used for gene inactivation of the MG1363 adhE gene is depicted as a filled box (plasmid construction pKAS15 [see Materials and Methods]). Putative transcriptional terminators for orfB and adhE are shown as open circles.