FIG. 4.

Northern blot and primer extension analysis of the L. lactis adhE gene. (A) Northern blot analysis of total RNA from L. lactis MG1363 grown in fermentors. Lane 1, aerobic growth in GM17; lane 2, anaerobic growth in GM17; lane 3, aerobic growth in GalM17; lane 4, anaerobic growth in GalM17. The size of the adhE transcript is shown on the left in kilobases. (B and C) Primer extension carried out with primers mgadhe-PE1 (B) and mgadhe-PE2 (C) on total RNA of strain MG1363. Lanes 1 to 4, MG1363 grown as described for panel A. A sequence ladder was run, in each case, with the corresponding primer. The adhE transcription start site is indicated with a box in the sequence line. Extension products are indicated with arrows.