FIG. 1.
DNA sequence of the wbEcO7 promoter region. The sequence has been published previously (GenBank accession no. U23775 [26]). The end of galF and the beginning of rlmB are indicated. The diverging arrows indicate the locations of inverted repeats at the end of galF that presumably are involved in transcription termination. The −35 and −10 sequences of the wbEcO7 promoter are in boldface and double underlined. The underlined sequences overlapping the −35 and −10 regions correspond to the −35 and −10 sequences of a putative second promoter. The stars above boldface G and A letters denote alternative sites of initiation of transcription according to the RNase protection data shown in Fig. 3, although all but the G at +1 probably correspond to sites of RNA processing (see the text). The endpoint of the DNA insert in pCM197 that lacks the −35 sequences is indicated by the labeled arrow. The nucleotides of the JUMPStart sequence are in boldface and underlined with dots. The ops1 and ops2 subsequences are indicated in lowercase. The asterisks indicate the 5′ ends of the RNase-protected fragments.