Figure 6.

OLE-enhanced mitochondrial biogenesis was related to the LKB1-PGC-1α pathway in OA-induced cells. Relative mRNA expression of mitochondrial-biogenesis-related genes (A) PGC-1α and (B) Tfam. (C,D) Representative band of t-AMPK and relative expression of t-AMPK to GAPDH. (E) Relative mRNA expression of LKB1. (F) LKB1 recruitment to the PGC-1α promoter region in OLE-JGF-treated mice liver cells detected by ChIP assay. IgG was used as a negative control. Two-tailed unpaired t-test was used to analyze the mRNA expression of PGC-1α and LKB1. Data are presented as mean ± SEM (n = 4–6). * p < 0.05 and ** p < 0.01.