| Time, min | A, % | B, % | Flow, mL/min |
| 0.00 | 97.00 | 3.00 | 0.900 |
| 1.50 | 0.00 | 100.00 | 0.900 |
| 1.90 | 0.00 | 100.00 | 0.900 |
| 2.00 | 97.00 | 3.00 | 0.900 |
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