FIG. 1.

Immunological evidence for expression of NhaA in yeast membranes. Cellular homogenization, centrifugation to resolve soluble and membrane fractions, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blot analysis were performed as described in Materials and Methods. Lanes 1, purified NhaA protein (2 μg); lanes 2, soluble fraction from strain RS-1045 (expressing NhaA) (30 μg of protein); lanes 3, membrane fraction from strain RS-1045 (expressing NhaA) (30 μg of protein); lanes 4, membrane fraction from control strain RS-997 (not expressing NhaA) (30 μg of protein). (Left) Coomassie blue-stained gel. (Right) Western immunoblot with antibody against the NhaA protein.