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Dual subcellular localization of NhaA expressed in yeast. Membranes from strain RS-1045 (expressing NhaA) were fractionated by equilibrium sucrose gradient centrifugation as described in Materials and Methods. The top of the gradient is fraction 1. Aliquots (15 μl) of the fractions were analyzed by Western blotting with antibodies against either NhaA (A) or the plasma membrane marker Pma1 (B). The sucrose concentration of every fraction is also shown (C).
