FIG. 5.

Effect of a functional vacuolar H⁺ ATPase on lithium accumulation and efflux kinetics. Cells from strains RS-1178 (ENA1-4 tfp1 nhaA) (squares) and RS-1176 (ENA1-4 TFP1 nhaA) (triangles) were grown on medium with 50 mM LiCl and washed, and lithium efflux was measured as described in Materials and Methods. Values are averages from three experiments and the error bars indicate standard deviations.