Identification of oriV downstream of the nar operon. Restriction maps of the nar region (shaded bars) and of the plasmids used to localize a replicative oriV. Genes narG, narH, narJ, and narI are labeled. The kat gene used for their selection in Thermus strains is shown by black bars. The approximate position of the nar promoter (Pnar) is shown. Restriction enzyme abbreviations: E, EcoRI; K, KpnI; P, PstI; S, SalI; Sm, SmaI; X, XhoI.