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pNIT9kat replicates in Thermus strains. (A) Agarose gel electrophoresis of EcoRI-digested pNIT9kat purified from E. coli (lane 1) and EcoRI-digested plasmid fraction from T. thermophilus HB8 transformed with pNIT9kat (lane 2). (B) Parallel Southern blot labeled with probe B. Lanes are as in panel A. HindIII-digested DNAs from λ and φ29 phages were used as size markers (lane M).
