TABLE 2.
Identification of a replicative ori downstream of the nar operona
| Host | Level of Kmr colonies in the presence of plasmid:
|
|||||
|---|---|---|---|---|---|---|
| pMK18 | pNIT9kat | pNIT5kat1 | pNIT5kat1XS | pNIT5kat2 | pNIT10kat | |
| HB8 | +++ | +++ | ++++ | ++++ | ++ | − |
| HB27Camr::nar | ++++ | +++ | ++++ | ++++ | ++ | − |
| HB27Camr | ++++ | − | − | − | − | − |
| BPL7 | ++ | − | − | − | − | − |
| E. coli TG1 | ++++ | +++ | +++ | +++ | +++ | |
a
Data represent the results of transformation of Thermus strains with plasmids purified from E. coli and the results of transformation of E. coli with the same plasmids isolated from Thermus strains. pMK18 was used as a control for transformation efficiency. Number of colonies: −, none; ++, 50 to 100; +++, 100 to 500; ++++, >500.