Figure 1.

Effect of DSF on viability and expression of ATP7B and PD-L1 of A549 cells. (A) Viability of A549 cells treated with DSF and/or Cu²⁺ was detected by Cell Counting Kit-8 assay. (B) mRNA expression of ATP7B and PD-L1 in A549 cells treated with DSF and/or Cu²⁺ was detected by reverse transcription-quantitative PCR. (C) Expression of ATP7B and PD-L1 in A549 cells treated with DSF was detected by western blot. (D) The protein expression of ATP7B and PD-L1 in A549 cells treated with DSF and Cu²⁺ was detected by western blot. ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. A549. DSF, disulfiram; ATP7B, ATPase copper-transporting β; PD-L1, programmed death-ligand 1.